Recombinant Human CDC23 Protein, GST-Tagged

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Recombinant Human CDC23 Protein, GST-Tagged

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Cat.No. : CDC23-0912H
Product Overview : Human CDC23 full-length ORF (AAH17713, 1 a.a. - 591 a.a.) recombinant protein with GST-tag at N-terminal.
Description : The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 90.75 kDa
AA Sequence : MVPVAVTAAV APVLSINSDF SDLREIKKQL LLIAGLTRER GLLHSSKWSA ELAFSLPALP LAELQPPPPI TEEDAQDMDA YTLAKAYFDV KEYDRAAHFL HGCNSKKAYF LYMYSRYLSG EKKKDDETVD SLGPLEKGQV KNEALRELRV ELSKKHQARE LDGFGLYLYG VVLRKLDLVK EAIDVFVEAT HVLPLHWGAW LELCNLITDK EMLKFLSLPD TWMKEFFLAH IYTELQLIEE ALQKYQNLID VGFSKSSYIV SQIAVAYHNI RDIDKALSIF NELRKQDPYR IENMDTFSNL LYVRSMKSEL SYLAHNLCEI DKYRVETCCV IGNYYSLRSQ HEKAALYFQR ALKLNPRYLG AWTLMGHEYM EMKNTSAAIQ AYRHAIEVNK RDYRAWYGLG QTYEILKMPF YCLYYYRRAH QLRPNDSRML VALGECYEKL NQLVEAKKCY WRAYAVGDVE KMALVKLAKL HEQLTESEQA AQCYIKYIQD IYSCGEIVEH LEESTAFRYL AQYYFKCKLW DEASTCAQKC CAFNDTREEG KALLRQILQL RNQGETPTTE VPAPFFLPAS LSANNTPTRR VSPLNLSSVT P
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : CDC23 cell division cycle 23 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol : CDC23
Synonyms : CDC23; cell division cycle 23 homolog (S. cerevisiae); CDC23 (cell division cycle 23, yeast, homolog); cell division cycle protein 23 homolog; ANAPC8; anaphase promoting complex subunit 8; APC8; CUT23; cyclosome subunit 8; anaphase-promoting complex subunit 8;
Gene ID : 8697
mRNA Refseq : NM_004661
Protein Refseq : NP_004652
MIM : 603462
UniProt ID : Q9UJX2

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