Recombinant Human CDC25C Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CDC25C-5300H
• Product Overview: CDC25C MS Standard C13 and N15-labeled recombinant protein (NP_001781) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 53.3 kDa
• AA Sequence: MSTELFSSTREEGSSGSGPSFRSNQ RKMLNLLLERDTSFTVCPDVPRTPV GKFLGDSANLSILSGGTPKCCLDLS NLSSGEITATQLTTSADLDETGHLD SSGLQEVHLAGMNHDQHLMKCSPAQ LLCSTPNGLDRGHRKRDAMCSSSAN KENDNGNLVDSEMKYLGSPITTVPK LDKNPNLGEDQAEEISDELMEFSLK DQEAKVSRSGLYRSPSMPENLNRPR LKQVEKFKDNTIPDKVKKKYFSGQG KLRKGLCLKKTVSLCDITITQMLEE DSNQGHLIGDFSKVCALPTVSGKHQ DLKYVNPETVAALLSGKFQGLIEKF YVIDCRYPYEYLGGHIQGALNLYSQ EELFNFFLKKPIVPLDTQKRIIIVF HCEFSSERGPRMCRCLREEDRSLNQ YPALYYPELYILKGGYRDFFPEYME LCEPQSYCPMHHQDHKTELLRCRSQ SKVQEGERQLREQIALLVKDMSPTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CDC25C cell division cycle 25C [ Homo sapiens (human) ]
• Official Symbol: CDC25C
• Synonyms: CDC25C; cell division cycle 25 homolog C (S. pombe); CDC25, cell division cycle 25 homolog C (S. cerevisiae), cell division cycle 25C; M-phase inducer phosphatase 3; PPP1R60; protein phosphatase 1; regulatory subunit 60; mitosis inducer CDC25; cell division cycle 25C; phosphotyrosine phosphatase; dual specificity phosphatase CDC25C; protein phosphatase 1, regulatory subunit 60; CDC25
• Gene ID: 995
• mRNA Refseq: NM_001790
• Protein Refseq: NP_001781
• MIM: 157680
• UniProt ID: P30307