Recombinant Human CDC42(Q61L) protein, His-tagged

• Cat.No.: CDC42-2637H
• Product Overview: Recombinant Human CDC42 fused with a histidine tag in the amino-terminus was expressed in E. coli.

Specification

• Description: The constitutively active form of human Cdc42 protein contains a glutamine to leucine substitution at residue 61. The common name for this mutant is Cdc42(Q61L) or L61Cdc42. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of Cdc42, hence the protein is always in the active, GTP-bound, state. A similar mutation in Ras (Ras(Q61L)) behaves as an oncogenic mutation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran.
• Molecular Mass: ~25 kDa
• Purity: >70% pure
• Applications: Cdc42 effector and GAP identification and binding studies; Activation of Cdc42 effectors in vitro and in vivo; Stimulation of Cdc42 phenotype by microinjection into cells

Gene Information

• Gene Name: CDC42 cell division cycle 42 (GTP binding protein, 25kDa) [ Homo sapiens ]
• Official Symbol: CDC42
• Synonyms: CDC42; cell division cycle 42 (GTP binding protein, 25kDa); cell division cycle 42 (GTP binding protein, 25kD); cell division control protein 42 homolog; CDC42Hs; G25K; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
• Gene ID: 998
• mRNA Refseq: NM_001039802
• Protein Refseq: NP_001034891
• MIM: 116952
• UniProt ID: P60953
• Chromosome Location: 1p36.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem
• Function: GTP binding; GTP-dependent protein binding; GTPase activity; GTPase activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein binding; thioesterase binding