Recombinant Human CDH2 Protein, His-tagged

• Cat.No.: CDH2-001H
• Product Overview: Recombinant Human CDH2 Protein with His tag was expressed in E. coli.

Specification

• Description: Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~62 kDa
• AA Sequence: DWVIPPINLPENSRGPFPQELVRIR SDRDKNLSLRYSVTGPGADQPPTGI FIINPISGQLSVTKPLDREQIARFH LRAHAVDINGNQVENPIDIVINVID MNDNRPEFLHQVWNGTVPEGSKPGT YVMTVTAIDADDPNALNGMLRYRIV SQAPSTPSPNMFTINNETGDIITVA AGLDREKVQQYTLIIQATDMEGNPT YGLSNTATAVITVTDVNDNPPEFTA MTFYGEVPENRVDIIVANLTVTDKD QPHTPAWNAVYRISGGDPTGRFAIQ TDPNSNDGLVTVVKPIDFETNRMFV LTVAAENQVPLAKGIQHPPQSTATV SVTVIDVNENPYFAPNPKIIRQEEG LHAGTMLTTFTAQDPDRYMQQNIRY TKLSDPANWLKIDPVNGQITTIAVL DRESPNVKNNIYNATFLASDNGIPP MSGTGTLQIYLLDINDNAPQVLPQE AETCETPDPNSINITALDYDIDPNA GPFAFDLPLSPVTIKRNWTITRLNG DFAQLNLKIKFLEAGIYEVPIIITD SGNPPKSNISILRVKVCQCDSNGDC TDVDRIVGAGLGTGA
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: CDH2 cadherin 2, type 1, N-cadherin (neuronal) [ Homo sapiens (human) ]
• Official Symbol: CDH2
• Synonyms: CDH2; cadherin 2, type 1, N-cadherin (neuronal); NCAD; cadherin-2; CD325; CDHN; N cadherin; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; CDw325
• Gene ID: 1000
• mRNA Refseq: NM_001792
• Protein Refseq: NP_001783
• MIM: 114020
• UniProt ID: P19022