"CDH3" Related Products

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Recombinant Human CDH3 protein, His/Fc-tagged

Cat.No.: CDH3-488H
Product Overview: Recombinant Human CDH3(Asp108 - Gly654) fused with His/Fc tag at C-terminal was expressed in NS0.
Source: NS0
Species: Human
Tag: His/Fc
Predicted N Terminal: Asp108
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1 x 10^5 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 30-60% will adhere after 1.5 hours at 37° C.Optimal concentration depends on cell type as well as the application or research objectives.
Molecular Mass: Predicted Molecular Mass: 87.4 kDa;SDS-PAGE: 120-130 kDa, under reducing conditions.
Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
Gene Name: CDH3 cadherin 3, type 1, P-cadherin (placental) [ Homo sapiens ]
Official Symbol: CDH3
Synonyms: CDH3; cadherin 3, type 1, P-cadherin (placental); cadherin 3, P cadherin (placental); cadherin-3; CDHP; PCAD; calcium-dependent adhesion protein, placental; HJMD;
Gene ID: 1001
mRNA Refseq: NM_001793
Protein Refseq: NP_001784
MIM: 114021
UniProt ID: P22223
Chromosome Location: 16q22.1
Pathway: Adherens junctions interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem;
Function: calcium ion binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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