Recombinant Human CDK1 & CCNE1 protein, His&GST-tagged

• Cat.No.: CDK1 & CCNE1-2209H
• Product Overview: Recombinant Human CDK1 & CCNE1 protein(NP_001777.1 & NP_001229.1)(Met1-Met297 & Met1-Ala410), fused with His&GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Met1-Met297 & Met1-Ala410
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0.
• Molecular Mass: The recombinant heterotrimer of human CDC2 & CCNE1 comprises 950 (522+428) amino acids and has a calculated molecular mass of 109.7 (60.4+49.3) kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CDK1 cyclin-dependent kinase 1 [ Homo sapiens ]
• Official Symbol: CDK1
• Synonyms: CDK1; cyclin-dependent kinase 1; CDC2, cell division cycle 2, G1 to S and G2 to M; CDC28A; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; CDC2; P34CDC2; MGC111195; DKFZp686L20222
• Gene ID: 983
• mRNA Refseq: NM_001170406
• Protein Refseq: NP_001163877
• MIM: 116940
• UniProt ID: P06493