Recombinant Human CDK2-CCNE1 Protein, Full Length, C-6×His and N-GST tagged

• Cat.No.: CDK2-CCNE1-01HFL
• Product Overview: Complex of recombinant, human full length cdk2 containing a C-terminal 6×His-tag, and recombinant, human full length cyclin E containing an N-terminal GST-tag.

Specification

• Description: The cyclin-dependent kinases (CDKs), are a family of serine threonine protein kinases, that control the cell division cycle. In mammals, nine different CDKs (cdk 1 to cdk 9) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk2 promotes the G1/S transition and progression through the S phase when bound to specific cyclins (E and A). Recent evidences indicate that CDK2 is involved in cell cycle independent functions such as DNA damage repair.
• Source: Insect cells
• Species: Human
• Tag: C-6×His and N-GST
• Protein length: Full length
• Form: Frozen solution
• Bio-activity: 1898 U/mg, where one unit of CDK2/cylinE1 activity is defined as 1nmol phosphate incorporated into 0.1 mg/mL histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: CDK2 MW = 34 kDa
  cyclinE1 MW = 74 kDa
• AA Sequence: CDK2: MENFQKVEKIGEGTYGVVYKARNKL TGEVVALKKIRLDTETEGVPSTAIR EISLLKELNHPNIVKLLDVIHTENK LYLVFEFLHQDLKKFMDASALTGIP LPLIKSYLFQLLQGLAFCHSHRVLH RDLKPQNLLINTEGAIKLADFGLAR AFGVPVRTYTHEVVTLWYRAPEILL GCKYYSTAVDIWSLGCIFAEMVTRR ALFPGDSEIDQLFRIFRTLGTPDEV VWPGVTSMPDYKPSFPKWARQDFSK VVPPLDEDGRSLLSQMLHYDPNKRI SAKAALAHPFFQDVTKPVPHLRLHH HHHH
  cyclinE1: MSPILGYWKIKGLVQPTRLLLEYLE EKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYI ADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSK LPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPK LVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLF QGPEFMPRERRERDAKERDTMKEDG GAEFSARSRKRKANVTVFLQDPDEE MAKIDRTARDQCGSQPWDNNAVCAD PCSLIPTPDKEDDDRVYPNSTCKPR IIAPSRGSPLPVLSWANREEVWKIM LNKEKTYLRDQHFLEQHPLLQPKMR AILLDWLMEVCEVYKLHRETFYLAQ DFFDRYMATQENVVKTLLQLIGISS LFIAAKLEEIYPPKLHQFAYVTDGA CSGDEILTMELMIMKALKWRLSPLT IVSWLNVYMQVAYLNDLHEVLLPQY PQQIFIQIAELLDLCVLDVDCLEFP YGILAASALYHFSSSELMQKVSGYQ WCDIENCVKWMVPFAMVIRETGSSK LKHFRGVADEDAHNIQTHRDSLDLL DKARAKKAMLSEQNRASPLPSGLLT PPQSGKKQSSGPEMA
• Purity: 77.3% by SDS-PAGE and Coomassie blue staining.
• Notes: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Stability: 1 year at -70 centigrade
• Storage: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.
• Concentration: 0.914 mg/mL
• Storage Buffer: 50 mM Tris/HCl pH7.5, 150 mM NaCl, 0.03% Brij-35, 0.1 mM EGTA, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol, 270 mM sucrose.

Gene Information

• Gene Name: CDK2 cyclin dependent kinase 2 [ Homo sapiens (human) ]
• Official Symbol: CDK2
• Synonyms: CDK2; cyclin dependent kinase 2; CDKN2; p33(CDK2); cyclin-dependent kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33 protein kinase; EC 2.7.11.22; CCNE; Ccne1; CCNE1; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; pCCNE1
• Gene ID: 1017
• mRNA Refseq: NM_052827
• Protein Refseq: NP_439892
• MIM: 116953
• UniProt ID: B4DDL9