Recombinant Human CDK3/CyclinE1, GST-tagged, Active

• Cat.No.: CDK3/CyclinE1-273H
• Product Overview: Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus inSf9insect cells using an N-terminal GST tag on both proteins.

Specification

• Cat. No.: CDK3/CyclinE1-273H
• Description: CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported.
• Source: Sf9 insect cells using baculovirus.
• Molecular Weight: ~60 kDa and CyclinE1 ~73 kDa.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CDK3 cyclin-dependent kinase 3 [ Homo sapiens ]CCNE1 cyclin E1 [ Homo sapiens ]
• Synonyms: CDK3; cyclin-dependent kinase 3; OTTHUMP00000206828; EC 2.7.11.22;Cell division protein kinase 3; CCNE1; cyclin E1; CCNE; cyclin E1; cyclin Es; cyclin Et; CCNE
• Gene ID: 1018898
• mRNA Refseq: NM_001258NM_001238
• Protein Refseq: NP_001249NP_001229
• MIM: 123828123837
• UniProt ID: Q00526P24864
• Chromosome Location: 17q22-qter19q12
• Pathway: Cell cycle; Pathways in cancer; Prostate cancer; Small cell lung cancer; p53 signaling pathway
• Function: ATP binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; transferase activity; androgen receptor binding; protein complex binding; transcription coactivator activity