Active Recombinant Human CDK5 Protein, GST-Tagged

• Cat.No.: CDK5-1021H
• Product Overview: Human CDK5 (NP_004926.1, 1 a.a. - 292 a.a.) full-length recombinant protein with GST tag co-expressed with CDK5R1 (NP_003876.1, 99 a.a. a.a. - 307 a.a.) expressed in Baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
• Source: Insect Cell
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: Modified Histone H1. ATP: 100 μM.
• Molecular Mass: 60 kDa
• Purity: 99 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CDK5 cyclin-dependent kinase 5 [ Homo sapiens ]
• Official Symbol: CDK5
• Synonyms: CDK5; cyclin-dependent kinase 5; PSSALRE; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit
• Gene ID: 1020
• mRNA Refseq: NM_001164410
• Protein Refseq: NP_001157882
• MIM: 123831
• UniProt ID: Q00535