Recombinant Human CDK5 Protein, His-tagged

• Cat.No.: CDK5-236H
• Product Overview: Recombinant Human CDK5(1-292aa) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Description: CDK5, also known as cyclin-dependent-like kinase 5 isoform 1, is a member of the cyclin dependent kinase family of serine/threonine kinases. It is present in numerous mammalian tissues including kidney, testes, and ovary. Its activity is detected almost exclusively in brain extracts. This is activated by association with a neuron-specific activator, p35 or its isoform p39. CDK5 is probably involved in the control of the cell cycle.
• Source: Insect cells
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 34.1kDa (298aa) 28-40KDa (SDS-PAGE under reducing conditions.)
• Protein length: 1-292aa
• AA Sequence: MQKYEKLEKI GEGTYGTVFK AKNRETHEIV ALKRVRLDDD DEGVPSSALR EICLLKELKH KNIVRLHDVL HSDKKLTLVF EFCDQDLKKY
  FDSCNGDLDP EIVKSFLFQL LKGLGFCHSR NVLHRDLKPQ NLLINRNGEL KLADFGLARA FGIPVRCYSA EVVTLWYRPP DVLFGAKLYS
  TSIDMWSAGC IFAELANAGR PLFPGNDVDD QLKRIFRLLG TPTEEQWPSM TKLPDYKPYP MYPATTSLVN VVPKLNATGR DLLQNLLKCN
  PVQRISAEEA LQHPYFSDFC PPHHHHHH
• Purity: > 90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Absorbance at 280nm)
• Publication:
  A cyclin-dependent kinase 5-derived peptide inhibits Cdk5/p25 activity and improves neurodegenerative phenotypes (2020)
  A Cdk5-derived peptide inhibits Cdk5/p25 activity and improves neurodegenerative phenotypes (2023)

Gene Information

• Gene Name: CDK5 cyclin-dependent kinase 5 [ Homo sapiens ]
• Official Symbol: CDK5
• Synonyms: CDK5; cyclin-dependent kinase 5; PSSALRE; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit
• Gene ID: 1020
• mRNA Refseq: NM_001164410
• Protein Refseq: NP_001157882
• MIM: 123831
• UniProt ID: Q00535