Recombinant Human CDK6/CycD3 Protein, GST/His-tagged

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Recombinant Human CDK6/CycD3 Protein, GST/His-tagged

CDK6/CycD3 Related Products

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Cat.No. : CDK6/CycD3-3549H
Product Overview : Recombinant Human CDK6/CycD3 Protein is produced by Sf9 insect cells expression system. Human CDK6, amino acids M1-A326, N-terminal GST fusion protein with a Thrombin cleavage site and human CycD3, amino acids M1-L292, N-terminally fused to HIS6-Thrombin cleavage site.
Source : Insect cells
Species : Human
Tag : GST/His
Form : 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20 % glycerol.
Bio-activity : Specific kinase activity (Pi transfer): 65 pmol/µg × min. ATP-KM: 43 µM.
Molecular Mass : GST-CDK6 (63,279 Da) and CycD3 (37,260 Da)
Protein length : CDK6 (M1-A326) and CycD3 (M1-L292)
AA Sequence : CDK6: MEKD GLCRADQQYE CVAEIGEGAY GKVFKARDLK NGGRFVALKR VRVQTGEEGM PLSTIREVAV LRHLETFEHP NVVRLFDVCT VSRTDRETKL TLVFEHVDQD LTTYLDKVPE PGVPTETIKD MMFQLLRGLD FLHSHRVVHR DLKPQNILVT SSGQIKLADF GLARIYSFQM ALTSVVVTLW YRAPEVLLQS SYATPVDLWS VGCIFAEMFR RKPLFRGSSY VDQLGKILDV IGLPGEEDWP RDVALPRQAF HSKSAQPIEK FVTDIDELGK DLLLKCLTFN PAKRISAYSA LSHPYFQDLE RCKENLDSHL PPSQNTSELN TA MEKDGLCRAD QQYECVAEIG EGAYGKVFKA RDLKNGGRFV ALKRVRVQTG EEGMPLSTIR EVAVLRHLET FEHPNVVRLF DVCTVSRTDR ETKLTLVFEH VDQDLTTYLD KVPEPGVPTE TIKDMMFQLL RGLDFLHSHR VVHRDLKPQN ILVTSSGQIK LADFGLARIY SFQMALTSVV VTLWYRAPEV LLQSSYATPV DLWSVGCIFA EMFRRKPLFR GSSDVDQLGK ILDVIGLPGE EDWPRDVALP RQAFHSKSAQ PIEKFVTDID ELGKDLLLKC LTFNPAKRIS AYSALSHPYF QDLERCKENL DSHLPPSQNT SELNTA
CycD3: MELLC CEGTRHAPRA GPDPRLLGDQ RVLQSLLRLE ERYVPRASYF QCVQREIKPH MRKMLAYWML EVCEEQRCEE EVFPLAMNYL DRYLSCVPTR KAQLQLLGAV CMLLASKLRE TTPLTIEKLC IYTDHAVSPR QLRDWEVLVL GKLKWDLAAV IAHDFLAFIL HRLSLPRDRQ ALVKKHAQTF LALCATDYTF AMYPPSMIAT GSIGAAVQGL GACSMSGDEL TELLAGITGT EVDCLRACQE QIEAALRESL REAAQTSSSP APKAPRGSSS QGPSQTSTPT DVTAIHL MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQR EIKPHMRKML AYWMLEVCEE QRCEEEVFPL AMNYLDRYLS CVPTRKAQLQ LLGAVCMLLA SKLRETTPLT IEKLCIYTDH AVSPRQLRDW EVLVLGKLKW DLAAVIAHDF LAFILHRLSL PRDRQALVKKHAQTFLALCA TDYTFAMYPP SMIATGSIGA AVQGLGACSM SGDELTELLA GITGTEVDCL RACQEQIEAA LRESLREASQ TSSSPAPKAP RGSSSQGPSQ TSTPTDVTAI HL
Storage : Store at -80 centigrade.
For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles.
Concentration : 0.738 µg/µl
Gene Name : CDK6 cyclin-dependent kinase 6 [ Homo sapiens ]
Official Symbol : CDK6
Synonyms : CDK6; cyclin-dependent kinase 6; PLSTIRE; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE; MGC59692;
Gene ID : 1021
mRNA Refseq : NM_001145306
Protein Refseq : NP_001138778
MIM : 603368
UniProt ID : Q00534

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