Recombinant Human CFB protein, His-tagged

• Cat.No.: CFB-141H
• Product Overview: Recombinant Human CFB(26-259aa) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 26-259 a.a.
• Description: CFB, also known as complement factor B, is a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 27.3kDa (245aa)
  28-40KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: ADPEFTPWSL ARPQGSCSLE GVEIKGGSFR LLQEGQALEY VCPSGFYPYP VQTRTCRSTG SWSTLKTQDQ KTVRKAECRA IHCPRPHDFE
  NGEYWPRSPY YNVSDEISFH CYDGYTLRGS ANRTCQVNGR WSGQTAICDN GAGYCSNPGI PIGTRKVGSQ YRLEDSVTYH CSRGLTLRGS
  QRRTCQEGGS WSGTEPSCQD SFMYDTPQEV AEAFLSSLTE TIEGVDAEDG HGPGEQQKRH HHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: CFB complement factor B [ Homo sapiens ]
• Official Symbol: CFB
• Synonyms: CFB; complement factor B; B factor, properdin , BF, BFD; H2 Bf; C3 proactivator; C3/C5 convertase; C3 proaccelerator; properdin factor B; B-factor, properdin; glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein; BF; FB; BFD; GBG; CFAB; PBF2; AHUS4; FBI12; H2-Bf; FLJ54899
• Gene ID: 629
• mRNA Refseq: NM_001710
• Protein Refseq: NP_001701
• UniProt ID: P00751
• Chromosome Location: 6p21.3
• Pathway: Activation of C3 and C5, organism-specific biosystem; Alternative complement activation, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Initial triggering of complement, organism-specific biosystem
• Function: complement binding; peptidase activity; serine-type endopeptidase activity