Recombinant Human CFD Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CFD-3070H
• Product Overview: CFD MS Standard C13 and N15-labeled recombinant protein (NP_001919) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 27 kDa
• AA Sequence: MHSWERLAVLVLLGAAACAAPPRGR ILGGREAEAHARPYMASVQLNGAHL CGGVLVAEQWVLSAAHCLEDAADGK VQVLLGAHSLSQPEPSKRLYDVLRA VPHPDSQPDTIDHDLLLLQLSEKAT LGPAVRPLPWQRVDRDVAPGTLCDV AGWGIVNHAGRRPDSLQHVLLPVLD RATCNRRTHHDGAITERLMCAESNR RDSCKGDSGGPLVCGGVLEGVVTSG SRVCGNRKKPGIYTRVASYAAWIDS VLATRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CFD complement factor D [ Homo sapiens (human) ]
• Official Symbol: CFD
• Synonyms: CFD; complement factor D (adipsin); D component of complement (adipsin), DF, PFD, properdin factor D; complement factor D; ADN; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin); DF; PFD; ADIPSIN
• Gene ID: 1675
• mRNA Refseq: NM_001928
• Protein Refseq: NP_001919
• MIM: 134350
• UniProt ID: P00746