Recombinant Human CFH Protein, His-tagged

• Cat.No.: CFH-763H
• Product Overview: Recombinant Human CFH Protein (Glu19-Leu322) with His tag was expressed in E. coli.

Specification

• Description: This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 38.0 kDa; Accurate Molecular Mass: 38 kDa
• Protein length: Glu19-Leu322
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CFH complement factor H [ Homo sapiens (human) ]
• Official Symbol: CFH
• Synonyms: CFH; complement factor H; H factor 1 (complement), HF, HF1, HF2; age related maculopathy susceptibility 1; ARMS1; beta 1H; FHL1; H factor 2 (complement); HUS; beta-1H; factor H; factor H-like 1; beta-1-H-globulin; H factor 1 (complement); adrenomedullin binding protein; complement factor H, isoform b; age-related maculopathy susceptibility 1; FH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; CFHL3; MGC88246
• Gene ID: 3075
• mRNA Refseq: NM_000186
• Protein Refseq: NP_000177
• MIM: 134370
• UniProt ID: P08603

Reviews

11/16/2020

Exceptional protein expression optimization support, highly appreciated assistance.

07/31/2020

Precise protein quantification, ensures data accuracy in our research.

03/10/2018

Timely antibody validation, an invaluable asset for our laboratory.

Q&As

What is the impact of CFH mutations on the risk of developing atypical hemolytic uremic syndrome (aHUS)?

Mutations in CFH increase the risk of aHUS, affecting complement regulation and leading to kidney damage.

How does CFH (Complement Factor H) regulate the complement system and prevent overactivation?

CFH controls the complement system, preventing its overactivation and protecting against immune damage.

How does CFH influence the immune response to bacterial infections?

CFH modulates the immune response to bacterial infections, balancing defense mechanisms.

How does CFH deficiency contribute to the development of age-related macular degeneration (AMD)?

CFH deficiency is linked to AMD, due to impaired regulation of the complement pathway in the retina.

What role does CFH play in protecting host cells from complement-mediated damage?

CFH shields host cells from complement-mediated injury, maintaining tissue integrity.

What are the mechanisms by which CFH modulates inflammation and immune homeostasis?

CFH regulates inflammation and maintains immune balance, crucial for preventing excessive immune responses.

How do alterations in CFH expression or function affect renal function and the development of kidney diseases?

Changes in CFH function can impact kidney health, potentially leading to renal diseases due to altered complement activity.