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Recombinant Human CHAT HEK293T cell lysate

Cat.No.: CHAT-175HCL
Product Overview: Human CHAT derived in Human HEK293T cell line. The cell lysate is provided in RIPA buffer.
Description: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Source: Human HEK293T cell line
Species: Human
Molecular Mass: 82.4 kDa
Components: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer;1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer;1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT).
Preparation method: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping.
Gene Name: CHAT choline O-acetyltransferase [ Homo sapiens ]
Official Symbol: CHAT
Synonyms: CHAT; choline O-acetyltransferase; choline acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; CMS1A; CMS1A2; CHOACTASE;
Gene ID: 1103
mRNA Refseq: NM_020549
Protein Refseq: NP_065574
MIM: 118490
UniProt ID: P28329
Chromosome Location: 10q11.2
Pathway: Acetylcholine Neurotransmitter Release Cycle, organism-specific biosystem; Acetylcholine Synthesis, organism-specific biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Neuronal System, organism-specific biosystem;
Function: choline O-acetyltransferase activity; choline binding; transferase activity, transferring acyl groups;

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