Recombinant Human CHD4 protein, His-tagged
Cat.No. : | CHD4-2434H |
Product Overview : | Recombinant Human CHD4 fused with His tag was expressed in Insect cells. CHD4 is a full-length cDNA coding for the human Mi-2 beta isoform. |
- Specification
- Gene Information
- Related Products
Description : | CHD4 is a member of a family of alleged chromodomain helicase-DNA-binding proteins. Biochemically, CHD4 is a component of the nucleosome transformation and deacetylase (NuRD) complex that takes part in transcription regulation. Autoantibodies targeting the CHD4 are a serologic feature of idiopathic inflammatory myopathies (IIM). In IIM Mi-2 antibodies are characterized by diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. Moreover, anti-CHD4 antibodies are related to dermatomyositis (frequency up to 31%) and have a great positive predictive value for this type of disease subset. Anti-CHD4 are the only defined myositis-specific autoantibodies clearly focused to a nuclear target. An additional slightly outstanding feature of Mi-2 antibodies relates to their frequency in children, which is similar to that in adults. |
Source : | Insect cells |
Species : | Human |
Tag : | His |
Form : | CHD4 is supplied in 20mM HEPES buffer pH-8.0 and 500mM NaCl. |
Molecular Mass : | 221,298 Dalton |
Purity : | Greater than 95% as determined by SDS-PAGE. |
Applications : | Western blot with myositis sera or monoclonal anti-hexa-His-tag antibody. Coating concentration: 0.3-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |
Storage : | Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.Avoid multiple freeze-thaw cycles. |
Gene Name : | CHD4 chromodomain helicase DNA binding protein 4 [ Homo sapiens ] |
Official Symbol : | CHD4 |
Synonyms : | CHD4; chromodomain helicase DNA binding protein 4; chromodomain-helicase-DNA-binding protein 4; Mi 2b; Mi2 BETA; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-BETA; DKFZp686E06161; |
Gene ID : | 1108 |
mRNA Refseq : | NM_001273 |
Protein Refseq : | NP_001264 |
MIM : | 603277 |
UniProt ID : | Q14839 |
Chromosome Location : | 12p13 |
Pathway : | Signaling events mediated by HDAC Class I, organism-specific biosystem; |
Function : | ATP binding; ATP-dependent DNA helicase activity; DNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; metal ion binding; nucleotide binding; protein binding; transcription factor binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
CHD4-1632M | Recombinant Mouse CHD4 Protein, His (Fc)-Avi-tagged | +Inquiry |
CHD4-1180H | Recombinant Human CHD4 Protein (1-239 aa), His-tagged | +Inquiry |
CHD4-772H | Recombinant Human CHD4 Protein, His-tagged | +Inquiry |
CHD4-5153H | Recombinant Human CHD4 protein, His&His-tagged | +Inquiry |
CHD4-0338H | Recombinant Human CHD4 Protein (Met1-Gln1912), C-His-tagged | +Inquiry |
◆ Lysates | ||
CHD4-346HCL | Recombinant Human CHD4 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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