Recombinant Human CHIT1 Protein, GST-Tagged

• Cat.No.: CHIT1-1238H
• Product Overview: Human CHIT1 full-length ORF (AAI03696.1, 1 a.a. - 466 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 78.1 kDa
• AA Sequence: MVRSVAWAGFMVLLMIPWGSAAKLV CYFTNWAQYRQGEARFLPKDLDPSL CTHLIYAFAGMTNHQLSTTEWNDET LYQEFNGLKKMNPKLKTLLAIGGWN FSTQKFTDMVATANNRQTFVNSAIR FLRKYSFDGLDLDWEYPGSQGSPAV DKERFTTLVQDLANAFQQEAQTSGK ERLLLSAAVPAGQTYVDAGYEVDKI AQNLDFVNLMAYDFHGSWEKVTGHN SPLYKRQEESGAAASLNVDAAVQQW LQKGTPASKLILGMPTYGRSFTLAS SSDTRVGAPATGSGTPGPFTKEGGM LAYYEVCSWKGATKQRIQDQKVPYI FRDNQWVGFDDVESFKTKVSYLKQK GLGGAMVWALDLDDFAGFSCNQGRY PLIQTLRQELSLPYLPSGTPELEVP KPGQPSEPEHGPSPGQDTFCQGKAD GLYPNPRERSSFYSCAGGRLFQQSC PTGLVFSNSCKCCTWN
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CHIT1 chitinase 1 (chitotriosidase) [ Homo sapiens ]
• Official Symbol: CHIT1
• Synonyms: CHIT1; chitinase 1 (chitotriosidase); chitotriosidase-1; CHI3; CHIT; chitinase-1; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; CHITD; FLJ00314; MGC125322
• Gene ID: 1118
• mRNA Refseq: NM_001256125
• Protein Refseq: NP_001243054
• MIM: 600031
• UniProt ID: Q13231