Active Recombinant Human CHIT1 protein, His-tagged

• Cat.No.: CHIT1-1257H
• Product Overview: Recombinant Human Chitinase-1/CHIT1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala22-Asn466) of human CHIT1 (Accession #NP_003456) fused with a 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ala22-Asn466
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its ability to cleave 4-Methylumbelliferyl-beta -D-N,N’,N”-triacetylchitotriose (4-MU-TACT). The specific activity is >43571 pmol/min/μg.
• Molecular Mass: 52 kDa
• Endotoxin: < 0.01 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: CHIT1 chitinase 1 (chitotriosidase) [ Homo sapiens ]
• Official Symbol: CHIT1
• Synonyms: CHIT1; chitinase 1 (chitotriosidase); chitotriosidase-1; CHI3; CHIT; chitinase-1; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; CHITD; FLJ00314; MGC125322
• Gene ID: 1118
• mRNA Refseq: NM_001256125
• Protein Refseq: NP_001243054
• MIM: 600031
• UniProt ID: Q13231