Active Recombinant Human CIT Protein, GST-tagged

• Cat.No.: CIT-1383H
• Product Overview: Human CIT (NP_009105.1, 1 a.a. - 449 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
• Source: Insect Cells
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Histone H3 peptide. ATP: 100 uM.
• Molecular Mass: 77 kDa
• Purity: 98 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CIT citron (rho-interacting, serine/threonine kinase 21) [ Homo sapiens ]
• Official Symbol: CIT
• Synonyms: CIT; citron (rho-interacting, serine/threonine kinase 21); citron Rho-interacting kinase; CRIK; KIAA0949; STK21; serine/threonine-protein kinase 21
• Gene ID: 11113
• mRNA Refseq: NM_001206999
• Protein Refseq: NP_001193928
• MIM: 605629
• UniProt ID: O14578