|Product Overview :||Recombinant Human CLEC10A protein (Gln61-His316), fused to human IgG1 Fc tag at N-terminus, was expressed in human 293 cells (HEK293).|
|Description :||C-type lectin domain family 10 member A (CLEC10A) is also known as C-type lectin superfamily member 14 (CLECSF14), Macrophage lectin 2, CD antigen CD301. CLEC10A/CD301 is a unique calcium-type (C-type) lectin, which expressed on Dendritic cells (DCs). CLEC10A/CD301 probable role in regulating adaptive and innate immune responses. CLEC10A/CD301 binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine (GalNAc), linked to serine or threonine. There are two homologues in mice: MGL1 and MGL2 (CD301a and CD301b).|
|Tag :||human IgG1 Fc|
|Form :||Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 55.7 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||256|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>92% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CLEC10A-348H :||
Human CLEC10A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
|Gene Name :||CLEC10A|
|Official Symbol :||CLEC10A|
|Synonyms :||CLEC10A; C-type lectin domain family 10, member A; C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 14 (macrophage derived) , CLECSF13, CLECSF14; C-type lectin domain family 10 member A; CD301; HML; HML2; macrophage lectin 2 (calcium dependent); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13 (macrophage-derived); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 14 (macrophage-derived); MGL; CLECSF13; CLECSF14|
|Gene ID :||10462|
|mRNA Refseq :||NM_006344|
|Protein Refseq :||NP_006335|
|UniProt ID :||Q8IUN9|