Recombinant Human CLPS Protein, His tagged

• Cat.No.: CLPS-08H
• Product Overview: Colipase (human, recombinant) protein consists of 105 amino acids, has a calculated molecular weight of 11.5 kDa, and a predicted N-terminus of Ala18 after signal peptide cleavage.

Specification

• Species: Human
• Source: Insect cells
• Tag: His
• Protein Length: 18-112 aa
• Description: The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 11.5 kDa
• Endotoxin: <1 EU/μg of the protein by the LAL
• Purity: ≥90% estimated by SDS-PAGE
• Stability: ≥ 1 year
• Storage: At -80 centigrade
• Storage Buffer: Lyophilized from sterile PBS, pH 7.0, 500 mM sodium chloride, and 10% glycerol
• Shipping: Dry ice in continental US; may vary elsewhere
• Reference: 1. Lowe, M.E. Structure and function of pancreatic lipase and colipase. Annu. Rev. Nutr. 17, 141-158 (1997). 2. Borgström, B., Wieloch, T., and Erlanson-Albertsson, C. Evidence for a pancreatic pro-colipase and its activation by trypsin. FEBS Lett. 108(2), 407-410 (1979). 3. van Tilbeurgh, H., Bezzine, S., Cambillau, C., et al. Colipase: Structure and interaction with pancreatic lipase. Biochim. Biophys. Acta 1441(2-3), 173-184 (1999).

Gene Information

• Gene Name: CLPS colipase [ Homo sapiens (human) ]
• Official Symbol: CLPS
• Synonyms: CLPS; colipase; colipase; colipase, pancreatic; pancreatic colipase preproprotein
• Gene ID: 1208
• mRNA Refseq: NM_001832
• Protein Refseq: NP_001823
• MIM: 120105
• UniProt ID: P04118