Recombinant Human COBRA1 Protein, GST-tagged

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Recombinant Human COBRA1 Protein, GST-tagged

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Cat.No. : COBRA1-1618H
Product Overview : Human COBRA1 full-length ORF ( AAH11892, 1 a.a. - 475 a.a.) recombinant protein with GST-tag at N-terminal.
Description : NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 77.99 kDa
AA Sequence : MPSLQPVVMC VMKHLPKVPE KKLKLVMADK ELYRACAVEV KRQIWQDNQA LFGDEVSPLL KQYILEKESA LFSTELSVLH NFFSPSPKTR RQGEVVQRLT RMVGKNVKLY DMVLQFLRTL FLRTRNVHYC TLRAELLMSL HDLDVGEICT VDPCHKFTWC LDACIRERFV DSKRARELQG FLDGVKKGQE QVLGDLSMIL CDPFAINTLA LSTVRHLQEL VGQETLPRDS PDLLLLLRLL ALGQGAWDMI DSQVFKEPKM EVELITRFLP MLMSFLVDDY TFNVDQKLPA EEKAPVSYPN TLPESFTKFL QEQRMACEVG LYYVLHITKQ RNKNALLRLL PGLVETFGDL AFGDIFLHLL TGNLALLADE FALEDFCSSL FDGFFLTASP RKENVHRHAL RLLIHLHPRV APSKLEALQK ALEPTGQSGE AVKELYSQLG EKLEQLDHRK PSPAQAAETP ALELPLPSVP APAPL
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing: :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : COBRA1 cofactor of BRCA1 [ Homo sapiens ]
Official Symbol : COBRA1
Synonyms : COBRA1; cofactor of BRCA1; negative elongation factor B; KIAA1182; NELF B; negative elongation factor protein B; NELFB; NELF-B; RP13-122B23.3; DKFZp586B0519;
Gene ID : 25920
mRNA Refseq : NM_015456
Protein Refseq : NP_056271
MIM : 611180
UniProt ID : Q8WX92

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