Recombinant Human COL17A1 Protein, His-tagged

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Recombinant Human COL17A1 Protein, His-tagged

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Cat.No. : COL17A1-329H
Product Overview : Recombinant Human COL17A1(Pro184~Pro375) fused with His tag at N-terminal was expressed in E. coli.
Description : This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form.
Source : E. coli
Species : Human
Tag : His
Form : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass : 21.5kDa
Protein length : Pro184~Pro375
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : > 97%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Figure :

Gene Name : COL17A1 collagen, type XVII, alpha 1 [ Homo sapiens ]
Official Symbol : COL17A1
Synonyms : COL17A1; collagen, type XVII, alpha 1; BPAG2; collagen alpha-1(XVII) chain; BP180; alpha 1 type XVII collagen; collagen XVII, alpha-1 polypeptide; 180 kDa bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BPA-2; LAD-1; BA16H23.2; FLJ60881; KIAA0204;
Gene ID : 1308
mRNA Refseq : NM_000494
Protein Refseq : NP_000485
MIM : 113811
UniProt ID : Q9UMD9

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