Recombinant Human COL3A1 protein, His & GST-tagged

• Cat.No.: COL3A1-7927H
• Product Overview: Recombinant Human COL3A1 aa. (Gly1059~Leu1466 (Accession # P02461)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 68 kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly1059~Leu1466 (Accession # P02461)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: COL3A1 collagen type III alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL3A1
• Synonyms: COL3A1; collagen type III alpha 1 chain; EDS4A; collagen alpha-1(III) chain; Ehlers-Danlos syndrome type IV, autosomal dominant; alpha-1 type III collagen; alpha1 (III) collagen; collagen, fetal; collagen, type III, alpha 1
• Gene ID: 1281
• mRNA Refseq: NM_000090.3
• Protein Refseq: NP_000081.1
• UniProt ID: P02461

Reviews

01/23/2020

This protein demonstrates great performance in WB assays, providing accurate and reliable results that are crucial for protein analysis.

05/08/2019

The COL3A1 protein is of exceptional quality, making it an ideal choice to meet my experimental needs.

07/27/2016

the COL3A1 protein has proven to be an invaluable tool in protein electron microscopy (EM) structure analysis.

Q&As

How can the study of COL3A1 protein aid in the development of personalized medicine for vEDS patients?

Understanding the specific genetic and protein alterations in vEDS patients can help tailor individualized treatment strategies based on the patient's unique molecular profile.

Can COL3A1 protein analysis be used for prenatal screening of vEDS?

Yes, analysis of COL3A1 protein levels in prenatal samples may provide valuable information for assessing the risk of vEDS in the developing fetus.

How can the measurement of COL3A1 protein levels aid in the early detection of vEDS?

Elevated or decreased levels of COL3A1 protein may serve as potential biomarkers for early detection and risk assessment of vEDS, especially in at-risk individuals or families.

What are the current challenges in the clinical application of COL3A1 protein analysis?

Challenges include the need for standardized assays and reference ranges for COL3A1 protein levels, as well as the interpretation of results in the context of individual patient variability.

Can the measurement of COL3A1 protein levels be used to monitor disease progression in vEDS patients?

Yes, monitoring changes in COL3A1 protein levels may provide insights into disease progression and response to treatment in vEDS patients.