Recombinant Human Complement Component 5a Receptor 1, His-tagged

• Cat.No.: C5AR1-4908H
• Product Overview: The recombinant protein is not further processed to remove the additional sequence.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: C5AR1 complement activation from the a-chain of complement C5. C5AR1 is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, C5AR1 protein expressed in E. coli with a 6 amino acid, N-terminal His·Tag sequence and 28 amino acid proprietary vector sequence necessary for expression.
• Biological Activity: Human recombinant C5AR1 is as active as that of serum derived C5AR1 based on enzyme release assay of myeloperoxidase. The current lot of EDS25;S20; = 1.36 nmolar.
• Molecular Weight: 74 amino acids
• Form: Lyophilized with no additives.
• Appearance: Lyophilized protein
• Endotoxin Level: <0.1 ng/μg
• Reconstitution: Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.
• Purity: ≥95% by SDS-PAGE
• Storage: Store at -20°C or -70°C as supplied. Stable for at least 1 year. After reconstition, store at -70°C.
• Pathways: Class A/1 (Rhodopsin-like receptors); Complement and Coagulation Cascades; G alpha (i) signalling events; GPCR downstream signaling; GPCR ligand binding; Neuroactive ligand-receptor interaction; Peptide GPCRs; Peptide ligand-binding receptors; Signaling by GPCR; Staphylococcus aureus infection

Gene Information

• Gene Name: C5AR1 complement component 5a receptor 1 [ Homo sapiens ]
• Official Symbol: C5AR1
• Synonyms: C5AR1; complement component 5a receptor 1; C5A; C5AR; C5R1; CD88; C5a anaphylatoxin chemotactic receptor; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1
• Gene ID: 728
• mRNA Refseq: NM_001736
• Protein Refseq: NP_001727
• MIM: 113995
• UniProt ID: P21730
• Chromosome Location: 19q13.3-q13.4
• Function: C5a anaphylatoxin receptor activity; G-protein coupled receptor activity; receptor activity; signal transducer activity