Recombinant Human CRBN/DDB1 Complex Protein, His&FLAG-tagged

• Cat.No.: CRBN&DDB1-920H
• Product Overview: Recombinant Human CRBN(NP_057386.2)(Met1-Leu442) was expressed in Insect Cells, with a polyhistidine tag at the N-terminus, constructed the plasmid 1; Recombinant Human DDB1(NP_001914.3) (Ser2-His1140) was expressed with a flag tag at the N-terminus, constructed the plasmid 2.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag&His
• Form: Lyophilized from sterile 20mM PB, 300mM NaCl, 10% Glycerol, pH 7.0.
• Molecular Mass: The recombinant heterodimer of human CRBN & DDB1 consists of 1601 (453+1148) amino acids and has a calculated molecular mass of 180.04 (52.06+127.98) KDa. It migrates as an approximately 119.6 kDa and 59.8 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CRBN cereblon [ Homo sapiens ]
• Official Symbol: CRBN
• Synonyms: CRBN; cereblon; mental retardation, non syndromic, autosomal recessive, 2A , MRT2A; protein cereblon; protein x 0001; MRT2; MRT2A; MGC27358; DKFZp781K0715
• Gene ID: 51185
• mRNA Refseq: NM_001173482
• Protein Refseq: NP_001166953
• MIM: 609262
• UniProt ID: Q96SW2
• Gene Name 2: DDB1 damage-specific DNA binding protein 1, 127kDa [ Homo sapiens ]
• Official Symbol 2: DDB1
• Synonyms 2: DDB1; damage-specific DNA binding protein 1, 127kDa; damage specific DNA binding protein 1 (127kD); DNA damage-binding protein 1; XPE; XAP-1; UV-DDB 1; DDB p127 subunit; XPE-binding factor; HBV X-associated protein 1; DNA damage-binding protein a; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; damage-specific DNA-binding protein 1; xeroderma pigmentosum group E-complementing protein; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
• Gene ID 2: 1642
• mRNA Refseq 2: NM_001923
• Protein Refseq 2: NP_001914
• MIM 2: 600045
• UniProt ID 2: Q16531