Active Native Human CKM Protein

• Cat.No.: CKM-932H
• Product Overview: Active Native Human CKM Protein was purified from human Skeletal Muscle. Isoenzyme Content: CK-MM: > 99% (Helena QuickGel®CK) CK-BB: < 1% CK-MB: < 1% Contaminants: GOT/AST: < 0.01% (Dimension® Clinical Chemistry System) LDH: < 0.01%

Specification

• Species: Human
• Source: Skeletal Muscle
• Description: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
• Form: Clear, colorless to red solution. Solution in 40 mM Tris, 2 mM DTT, 2 mM EDTA, pH 7.5 with 50% glycerol (v/v).
• Bio-activity: > 10,000 U/mL (Dimension Clinical Chemistry System). Specific Activity > 500 U/mg protein, One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37 centigrade.
• Purity: Highly Purified
• Applications: Clinical Chemistry; Cardiac
• Storage: At -20 centigrade
• Concentration: > 5.0 mg/mL (Coomassie)
• CAS: 9001-15-4
• EC: 2.7.3.2

Gene Information

• Gene Name: CKM creatine kinase, muscle [ Homo sapiens (human) ]
• Official Symbol: CKM
• Synonyms: CKM; creatine kinase, muscle; CKMM; creatine kinase M-type; creatine kinase-M; creatine kinase M chain; M-CK
• Gene ID: 1158
• mRNA Refseq: NM_001824
• Protein Refseq: NP_001815
• MIM: 123310
• UniProt ID: P06732