Recombinant Human CRH, His-tagged

• Cat.No.: CRH-107H
• Product Overview: A DNA sequence encoding the human FTO ( Q9C0B1-1 ) ( Lys 2 - Pro 505 ) was expressed, with a polyhistide tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-505 a.a.
• Description: Alpha-ketoglutarate-dependent dioxygenase FTO, also known as Fat mass and obesity-associated protein, FTO and KIAA1752, is a nucleus protein which belongs to the fto family. FTO is ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. FTO is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. FTO has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. It has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. FTO has no activity towards 1-methylguanine. It has no detectable activity towards double-stranded DNA. FTO requires molecular oxygen, alpha-ketoglutarate and iron. FTO contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. It contributes to the regulation of body size and body fat accumulation as well. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism.
• Form: Lyophilized from sterile 20mM Tris ，pH 8.0.
• Molecular Mass: The recombinant human FTO consisting of 515 amino acids and has a calculated molecular mass of 59.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Purity: > 92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃.
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CRH corticotropin releasing hormone [ Homo sapiens ]
• Official Symbol: CRH
• Synonyms: CRH; corticotropin releasing hormone; corticoliberin; corticotropin releasing factor; CRF; corticotropin-releasing factor
• Gene ID: 1392
• mRNA Refseq: NM_000756
• Protein Refseq: NP_000747
• MIM: 122560
• UniProt ID: P06850
• Chromosome Location: 8q13
• Pathway: Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Long-term depression, organism-specific biosystem; Long-term depression, conserved biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem
• Function: corticotropin-releasing hormone activity; corticotropin-releasing hormone receptor 1 binding; corticotropin-releasing hormone receptor 2 binding; hormone activity; neuropeptide hormone activity; protein binding; receptor binding