Recombinant Human cripto, EGF-CFC family member Protein, His&Flag tagged

• Cat.No.: CRIPTO-023H
• Product Overview: Recombinant human Cripto-1/TDGF1 protein (P13385) (Leu 31-Asp 150) with both His and Flag tag at the C-terminus was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 31-150aa
• Description: Teratocarcinoma-derived growth factor (TDGF)-1 gene encodes a protein known as cripto-1 (Cripto). GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. Cripto is first expressed in the forming mesoderm during gastrulation but later in development the expression is restricted to the truncus arteriosus of the developing heart. This suggests that Cripto mediates the progression of epiblastic cells that give rise to the mesoderm. In the adult animal it is expressed at low levels in the spleen, heart, lung and brain. Cripto overexpression is characteristic of human gastric and colorectal carcinomas.
• Tag: C-His&Flag
• Molecular Mass: 16.3 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: CRIPTO cripto, EGF-CFC family member [ Homo sapiens (human) ]
• Official Symbol: CRIPTO
• Synonyms: CRIPTO; cripto, EGF-CFC family member; CR; CR-1; CRGF; TDGF1; protein Cripto; cripto-1 growth factor; epidermal growth factor-like cripto protein CR1; teratocarcinoma-derived growth factor 1
• Gene ID: 6997
• mRNA Refseq: NM_001174136
• Protein Refseq: NP_001167607
• MIM: 187395
• UniProt ID: P13385