Recombinant Human CRLF2 protein, hFc-tagged

• Cat.No.: CRLF2-373H
• Product Overview: Recombinant Human CRLF2 protein(Q9HC73-1)(Gly25-Lys231), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Gly25-Lys231
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CRLF2 cytokine receptor-like factor 2 [ Homo sapiens ]
• Official Symbol: CRLF2
• Synonyms: CRLF2; cytokine receptor-like factor 2; CRL2; TSLPR; IL-XR; TSLP receptor; P2RY8/CRLF2 fusion; cytokine receptor-like 2; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin receptor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor; CRLF2Y
• Gene ID: 64109
• mRNA Refseq: NM_001012288
• Protein Refseq: NP_001012288
• MIM: 300357
• UniProt ID: Q9HC73