Recombinant Human CROT protein(474 Leu/Val), His-tagged

• Cat.No.: CROT-2507H
• Product Overview: Recombinant Human CROT protein(Q9UKG9) (Met 1-Leu 612, 474 Leu/Val) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-612 aa, 474 Leu/Val
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol.
• Molecular Mass: The recombinant human CROT consists of 623 amino acids and predicts a molecular mass of 71.5 kDa. The apparent molecular mass of rhCROT is apximately 65 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001137407.1
• Unit ID: Q9UKG9
• Unit ID-Weblink: http://www.uniprot.org/uniprot/Q9UKG9

Gene Information

• Gene Name: CROT carnitine O-octanoyltransferase [ Homo sapiens ]
• Official Symbol: CROT
• Synonyms: CROT; carnitine O-octanoyltransferase; peroxisomal carnitine O-octanoyltransferase; COT; peroxisomal carnitine acyltransferase
• Gene ID: 54677
• mRNA Refseq: NM_001143935
• protein Refseq: NP_001137407
• MIM: 606090