Recombinant Human CRYBA4 Protein, GST-tagged

• Cat.No.: CRYBA4-1931H
• Product Overview: Human CRYBA4 full-length ORF ( NP_001877.1, 1 a.a. - 196 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 48.8 kDa
• AA Sequence: MTLQCTKSAGPWKMVVWDEDGFQGR RHEFTAECPSVLELGFETVRSLKVL SGAWVGFEHAGFQGQQYILERGEYP SWDAWGGNTAYPAERLTSFRPAACA NHRDSRLTIFEQENFLGKKGELSDD YPSLQAMGWEGNEVGSFHVHSGAWV CSQFPGYRGFQYVLECDHHSGDYKH FREWGSHAPTFQVQSIRRIQQ
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CRYBA4 crystallin, beta A4 [ Homo sapiens ]
• Official Symbol: CRYBA4
• Synonyms: CRYBA4; crystallin, beta A4; beta-crystallin A4; beta-A4 crystallin; eye lens structural protein; crystallin, beta polypeptide A4; MCOPCT4
• Gene ID: 1413
• mRNA Refseq: NM_001886
• Protein Refseq: NP_001877
• MIM: 123631
• UniProt ID: P53673