Recombinant Human CSF3R Protein, Glu25-Pro621

• Cat.No.: CSF3R-12H
• Product Overview: Recombinant Human CSF3R Protein (Glu25-Pro621) without tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Protein Length: Glu25-Pro621
• Description: The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.
• Bio-activity: Measured by its ability to inhibit the G-CSF-induced proliferation of NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1-4 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF.
• Molecular Mass: Predicted Molecular Mass 66.5 kDa
  SDS-PAGE 95-105 kDa, reducing conditions
• Endotoxin: <0.01 EU/μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• N-terminal Sequence Analysis: Glu25
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CSF3R colony stimulating factor 3 receptor (granulocyte) [ Homo sapiens (human) ]
• Official Symbol: CSF3R
• Synonyms: CSF3R; colony stimulating factor 3 receptor (granulocyte); CD114; granulocyte colony-stimulating factor receptor; GCSFR; G-CSF-R; CD114 antigen; G-CSF receptor
• Gene ID: 1441
• mRNA Refseq: NM_000760
• Protein Refseq: NP_000751
• MIM: 138971
• UniProt ID: Q99062