Recombinant Human CSK9 Protein (31-692), C-His-Avi-Tagged, Biotin-Labeled

• Cat.No.: PCSK9-13H
• Product Overview: Recombinant human PCSK9 (proprotein convertase subtilisin/kexin type 9), encompassing amino acids 31-692(end). This construct contains the proprotein PCSK9 at the N-terminal encompassing amino acids 31-152. At the C-terminal this construct contains a His-tag (6×His) followed by an Avi-tag. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.
  PCSK9 is autocleaved to the ~14 kDa prodomain (a.a. 31-152) and the ~60 kDa mature form (a.a. 153-692(end)), which run at higher apparent MW by SDS-PAGE.

Specification

• Description: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Form: Aqueous buffer solution
• Molecular Mass: 74 kDa
• Protein Length: 31-692 (end)
• Purity: ≥ 90%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage: At least 6 months at –80 centigrade.
• Storage Buffer: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
• Shipping: At -80 centigrade
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: PCSK9 proprotein convertase subtilisin/kexin type 9 [ Homo sapiens (human) ]
• Official Symbol: PCSK9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3; proprotein convertase subtilisin/kexin type 9; convertase subtilisin/kexin type 9 preproprotein; neural apoptosis regulated convertase 1; subtilisin/kexin-like protease PC9; EC 3.4.21.-
• Gene ID: 255738
• mRNA Refseq: NM_174936
• Protein Refseq: NP_777596
• MIM: 607786
• UniProt ID: Q8NBP7