Active Recombinant Human CSTB protein, His-tagged

• Cat.No.: CSTB-204H
• Product Overview: Recombinant Human CSTB protein(P04080)(Met2-Phe98), fused with His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM Tris, 50mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is < 15 nM.
• Molecular Mass: The recombinant human CSTB consisting of 108 amino acids and has a calculated molecular mass of 12.5 kDa. It migrates as an approximately 15 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met2-Phe98
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CSTB cystatin B (stefin B) [ Homo sapiens ]
• Official Symbol: CSTB
• Synonyms: CSTB; cystatin B (stefin B); EPM1, STFB; cystatin-B; CST6; PME; CPI-B; liver thiol proteinase inhibitor; ULD; EPM1; STFB; EPM1A
• Gene ID: 1476
• mRNA Refseq: NM_000100
• Protein Refseq: NP_000091
• MIM: 601145
• UniProt ID: P04080