Recombinant Human CTNNB1 protein, His-tagged

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Recombinant Human CTNNB1 protein, His-tagged

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Cat.No. : CTNNB1-5747H
Product Overview : Recombinant Human CTNNB1 aa. (Cys439~Ser675 (Accession # P35222)) fused with N-terminal His tag was produced in E. coli cells.
Description : The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 27.7kDa
Protein length : Cys439~Ser675 (Accession # P35222)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 7.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
SDS-PAGE :

Gene Name : CTNNB1 catenin beta 1 [ Homo sapiens (human) ]
Official Symbol : CTNNB1
Synonyms : CTNNB1; catenin beta 1; CTNNB; MRD19; armadillo; catenin beta-1; catenin (cadherin-associated protein), beta 1, 88kDa
Gene ID : 1499
mRNA Refseq : NM_001098209.1
Protein Refseq : NP_001091679.1
UniProt ID : P35222
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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