Recombinant Human CTNNB1 protein(Met1-Leu781), His&GST-tagged

• Cat.No.: CTNNB1-3891H
• Product Overview: Recombinant Human CTNNB1 (P35222-1) (Met 1-Leu 781) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Protein length: Met1-Leu781
• Form: Lyophilized from sterile 50mM Tris, 150mM NaCl, 25% glycerol, pH 8.0, 0.1mM EDTA, 1mM TCEP, 0.4mM PMSF, 0.5mM GSH Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa [ Homo sapiens ]
• Official Symbol: CTNNB1
• Synonyms: CTNNB1; catenin (cadherin-associated protein), beta 1, 88kDa; catenin (cadherin associated protein), beta 1 (88kD) , CTNNB; catenin beta-1; beta catenin; CTNNB; FLJ25606; FLJ37923; DKFZp686D02253
• Gene ID: 1499
• mRNA Refseq: NM_001098209
• Protein Refseq: NP_001091679
• MIM: 116806
• UniProt ID: P35222

Reviews

03/27/2023

Not only does it provide reliable and consistent results due to its high quality and purity, but it also holds significant value in understanding cellular processes and disease mechanisms.

07/28/2017

the CTNNB1 protein offers numerous advantages in trials and research experiments.

06/24/2016

This commitment to excellence ensures that researchers are provided with the most advanced and reliable tools for their investigations.

Q&As

Is the CTNNB1 protein involved in the progression of pancreatic cancer?

Yes, CTNNB1 is implicated in the development and progression of pancreatic cancer, with its dysregulated activity contributing to tumor growth, invasion, and metastasis.

How is the CTNNB1 protein associated with hepatocellular carcinoma (HCC)?

Mutations in the CTNNB1 gene lead to the stabilization and accumulation of β-catenin, contributing to the development of HCC, making it a potential biomarker for the diagnosis and prognosis of HCC.

How is the CTNNB1 protein linked to the prognosis of gastric cancer patients?

Studies have shown that CTNNB1 expression levels can serve as an independent prognostic factor in gastric cancer, with high CTNNB1 expression correlating with poor patient outcomes.

Are there any targeted therapies specifically aimed at CTNNB1 in cancer treatment?

While there are no direct CTNNB1-targeted therapies available, various drugs targeting the Wnt signaling pathway are being developed and tested, which indirectly impact CTNNB1 activity in cancer cells.

How is the expression of CTNNB1 in breast cancer cells associated with patient survival?

High expression levels of CTNNB1 in breast cancer cells have been linked to poor prognosis and shorter overall survival, indicating its potential as a prognostic marker for breast cancer.