Recombinant Human CUL3/RBX1, GST-tagged

• Cat.No.: CUL3-240H
• Product Overview: Recombinant Human CUL3/RBX1(a.a: CUL3: 2-768(end); Rnx1: 1-108(end)) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 2-768;1-108 a.a.
• Description: Complex of human cullin-3 (CUL3) with human RING box protein 1 (Rbx1), also known as E3 ubiquitin-protein ligase RBX1. CUL3 (a.a. 2-768(end) with N-terminal GSTtag, 117 kDa) is co-expressed with Rbx1 (a.a. 1-108(end), 12 kDa) in a baculovirus infected Sf9 cell expression system. GenBank Accession Nos. are NM_003590 (CUL3) and NM_014248 (Rbx1).
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 1.6 mM glutathione.
• Molecular Mass: CUL3: 117 kDa; Rbx1: 12 kDa
• Purity: Purity: ≥72% (CUL3: 66%; RBX1: 6%)
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: At least 6 months at –80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1– 0.5%).
• Storage: At least 6 months at -80 centigrade
• Concentration: 0.13 mg/ml
• Warning: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Gene Information

• Gene Name: CUL3 cullin 3 [ Homo sapiens ]
• Official Symbol: CUL3
• Synonyms: CUL3; cullin 3; cullin-3; CUL-3; PHA2E; FLJ25665
• Gene ID: 8452
• mRNA Refseq: NM_003590
• Protein Refseq: NP_003581
• MIM: 603136
• UniProt ID: Q13618
• Chromosome Location: 2q36.2
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Class I MHC mediated antigen processing & amp
• Function: cyclin binding; protein binding; ubiquitin protein ligase binding; contributes_to ubiquitin-protein ligase activity