Recombinant Human CX3CR1 293 Cell Lysate

• Cat.No.: CX3CR1-7173HCL

Specification

• Description: Antigen standard for chemokine (C-X3-C motif) receptor 1 (CX3CR1), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: CX3CR1 chemokine (C-X3-C motif) receptor 1 [ Homo sapiens ]
• Official Symbol: CX3CR1
• Synonyms: CX3CR1; chemokine (C-X3-C motif) receptor 1; chemokine (C X3 C) receptor 1 , CMKBRL1, GPR13; CX3C chemokine receptor 1; CCRL1; CMKDR1; V28; CMK-BRL1; CMK-BRL-1; C-X3-C CKR-1; fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; chemokine (C-X3-C) receptor 1; beta chemokine receptor-like 1; chemokine (C-C) receptor-like 1; GPR13; GPRV28; CMKBRL1
• Gene ID: 1524
• mRNA Refseq: NM_001171171
• Protein Refseq: NP_001164642
• MIM: 601470
• UniProt ID: P49238
• Chromosome Location: 3p21.3
• Pathway: Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; GPCR ligand binding, organism-specific biosystem
• Function: C-X3-C chemokine receptor activity; G-protein coupled receptor activity; chemokine receptor activity; protein binding; receptor activity; signal transducer activity

Reviews

09/06/2016

The manufacturer's commitment to meeting regulatory requirements demonstrated its dedication to ethical research practices.

04/21/2016

The reliability of the product's quality and effectiveness from one batch to another instilled confidence in its usage.

02/08/2016

The product's adherence to regulatory standards aligned with our lab's commitment to responsible and transparent research.

Q&As

Are there therapeutic implications or potential targets related to CX3CR1 in immune-related disorders?

There are potential therapeutic implications related to CX3CR1 in immune-related disorders, making it a target of interest for drug development.

How does CX3CR1 contribute to the regulation of immune responses and cell migration?

CX3CR1 contributes to the regulation of immune responses and cell migration by responding to its ligand, fractalkine (CX3CL1).

In which immune cells is CX3CR1 predominantly expressed?

CX3CR1 is predominantly expressed in certain immune cells, including monocytes, macrophages, and certain subsets of T cells.

How does CX3CR1 influence the balance between immune tolerance and inflammation?

CX3CR1 plays a role in influencing the balance between immune tolerance and inflammation through its impact on immune cell function.

What diseases or conditions are associated with dysregulation or abnormal expression of CX3CR1?

Dysregulation or abnormal expression of CX3CR1 is associated with conditions such as atherosclerosis, neuroinflammatory disorders, and certain cancers.

Are there specific chemokines that bind to CX3CR1, and what is their role in immune modulation?

The ligand for CX3CR1, fractalkine (CX3CL1), is involved in immune modulation, influencing cell adhesion and migration.

What is the primary function of C-X3-C motif chemokine receptor 1 (CX3CR1) in the immune system?

C-X3-C motif chemokine receptor 1 (CX3CR1) primarily functions in the immune system, playing a role in immune responses and cell migration.