Recombinant Human Cyclin-Dependent Kinase 8, GST-tagged

• Cat.No.: CDK8-1003H
• Product Overview: Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for β-catenin-driven transformation and for expression of several β-catenin transcriptional targets.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 84 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol.
• Purity: > 80 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Diabetes pathways; Gene Expression; Generic Transcription Pathway; Transcriptional Regulation of White Adipocyte Differentiation

Gene Information

• Gene Name: CDK8 cyclin-dependent kinase 8 [ Homo sapiens ]
• Official Symbol: CDK8
• Synonyms: CDK8; cyclin-dependent kinase 8; K35; MGC126074; MGC126075; OTTHUMP00000018158; protein kinase K35; CDK8 protein kinase; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8; Cyclin-dependent kinase 8; EC 2.7.11.22; EC 2.7.11.23; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35
• Gene ID: 1024
• mRNA Refseq: NM_001260
• Protein Refseq: NP_001251
• MIM: 603184
• UniProt ID: P49336
• Chromosome Location: 13q12
• Function: ATP binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase activity