Recombinant Full Length Human CyclinK, GST-tagged

• Cat.No.: CCNK-6981H
• Product Overview: Recombinant full-length human CyclinK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full length
• Description: CyclinK is a member of the cyclin family which can regulate transcription through their association with and activation of cyclin-dependent kinases (CDK). The CDK/CyclinK complex can phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. CyclinK is associated with potent CTD kinase and CDK kinase activities in vitro and coimmunoprecipitates with POLR2A. CyclinK is a new member of the transcription cyclin family which may play a dual role in regulating CDK and RNA polymerase II activities.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~67 kDa
• Purity: >85%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.2 μg/μl

Gene Information

• Gene Name: CCNK cyclin K [ Homo sapiens ]
• Official Symbol: CCNK
• Synonyms: CCNK; cyclin K; cyclin-K; CPR4; MGC9113
• Gene ID: 8812
• mRNA Refseq: NM_001099402
• Protein Refseq: NP_001092872
• MIM: 603544
• UniProt ID: O75909
• Chromosome Location: 14q32
• Pathway: Direct p53 effectors, organism-specific biosystem
• Function: protein binding; protein kinase binding