| Cat.No. : |
CYLD-22H |
| Product Overview : |
Recombinant Human CYLD Protein with His tag was expressed in sf21 insect cell-baculovirus. |
| Description : |
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Source : |
Insect Cell |
| Species : |
Human |
| Tag : |
His |
| Bio-activity : |
Deubiquitylating Enzyme Assay: The activity of His-CYLD was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-CYLD was compared confirming the deubiquitylating activity of His-CYLD. |
| Molecular Mass : |
~110 kDa |
| AA Sequence : |
MSYYHHHHHH DYDIPTTENL YFQGAMGSSS GLWSQEKVTS PYWEERIFYL LLQECSVTDK QTQKLLKVPK GSIGQYIQDR SVGHSRIPSA KGKKNQIGLK ILEQPHAVLF VDEKDVVEIN EKFTELLLAI TNCEERFSLF KNRNRLSKGL QIDVGCPVKV QLRSGEEKFP GVVRFRGPLL AERTVSGIFF GVELLEEGRG QGFTDGVYQG KQLFQCDEDC GVFVALDKLE LIEDDDTALE SDYAGPGDTM QVELPPLEIN SRVSLKVGET IESGTVIFCD VLPGKESLGY FVGVDMDNPI GNWDGRFDGV QLCSFACVES TILLHINDII PALSESVTQE RRPPKLAFMS RGVGDKGSSS HNKPKATGST SDPGNRNRSE LFYTLNGSSV DSQPQSKSKN TWYIDEVAED PAKSLTEIST DFDRSSPPLQ PPPVNSLTTE NRFHSLPFSL TKMPNTNGSI GHSPLSLSAQ SVMEELNTAP VQESPPLAMP PGNSHGLEVG SLAEVKENPP FYGVIRWIGQ PPGLNEVLAG LELEDECAGC TDGTFRGTRY FTCALKKALF VKLKSCRPDS RFASLQPVSN QIERCNSLAF GGYLSEVVEE NTPPKMEKEG LEIMIGKKKG IQGHYNSCYL DSTLFCLFAF SSVLDTVLLR PKEKNDVEYY SETQELLRTE IVNPLRIYGY VCATKIMKLR KILEKVEAAS GFTSEEKDPE EFLNILFHHI LRVEPLLKIR SAGQKVQDCY FYQIFMEKNE KVGVPTIQQL LEWSFINSNL KFAEAPSCLI IQMPRFGKDF KLFKKIFPSL ELNITDLLED TPRQCRICGG LAMYECRECY DDPDISAGKI KQFCKTCNTQ VHLHPKRLNH KYNPVSLPKD LPDWDWRHGC IPCQNMELFA VLCIETSHYV AFVKYGKDDS AWLFFDSMAD RDGGQNGFNI PQVTPCPEVG EYLKMSLEDL HSLDSRRIQG CARRLLCDAY MCMYQSPTMS LYK |
| Stability : |
12 months at -70 centigrade; aliquot as required |
| Storage : |
At -70 centigrade |
| Concentration : |
0.5 mg/ml |
| Storage Buffer : |
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
| Purity : |
4-12% gradient SDS-PAGE
InstantBlue™ staining
Lane 1: MW markers
Lane 2: 1 µg His-CYLD
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.