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Active Recombinant Human CYLD Protein, His-tagged

Cat.No. : CYLD-22H
Product Overview : Recombinant Human CYLD Protein with His tag was expressed in sf21 insect cell-baculovirus.
  • Specification
  • Gene Information
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Description : This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Source : Insect Cell
Species : Human
Tag : His
Bio-activity : Deubiquitylating Enzyme Assay: The activity of His-CYLD was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-CYLD was compared confirming the deubiquitylating activity of His-CYLD.
Molecular Mass : ~110 kDa
AA Sequence : MSYYHHHHHHDYDIPTTENLYFQGA MGSSSGLWSQEKVTSPYWEERIFYL LLQECSVTDKQTQKLLKVPKGSIGQ YIQDRSVGHSRIPSAKGKKNQIGLK ILEQPHAVLFVDEKDVVEINEKFTE LLLAITNCEERFSLFKNRNRLSKGL QIDVGCPVKVQLRSGEEKFPGVVRF RGPLLAERTVSGIFFGVELLEEGRG QGFTDGVYQGKQLFQCDEDCGVFVA LDKLELIEDDDTALESDYAGPGDTM QVELPPLEINSRVSLKVGETIESGT VIFCDVLPGKESLGYFVGVDMDNPI GNWDGRFDGVQLCSFACVESTILLH INDIIPALSESVTQERRPPKLAFMS RGVGDKGSSSHNKPKATGSTSDPGN RNRSELFYTLNGSSVDSQPQSKSKN TWYIDEVAEDPAKSLTEISTDFDRS SPPLQPPPVNSLTTENRFHSLPFSL TKMPNTNGSIGHSPLSLSAQSVMEE LNTAPVQESPPLAMPPGNSHGLEVG SLAEVKENPPFYGVIRWIGQPPGLN EVLAGLELEDECAGCTDGTFRGTRY FTCALKKALFVKLKSCRPDSRFASL QPVSNQIERCNSLAFGGYLSEVVEE NTPPKMEKEGLEIMIGKKKGIQGHY NSCYLDSTLFCLFAFSSVLDTVLLR PKEKNDVEYYSETQELLRTEIVNPL RIYGYVCATKIMKLRKILEKVEAAS GFTSEEKDPEEFLNILFHHILRVEP LLKIRSAGQKVQDCYFYQIFMEKNE KVGVPTIQQLLEWSFINSNLKFAEA PSCLIIQMPRFGKDFKLFKKIFPSL ELNITDLLEDTPRQCRICGGLAMYE CRECYDDPDISAGKIKQFCKTCNTQ VHLHPKRLNHKYNPVSLPKDLPDWD WRHGCIPCQNMELFAVLCIETSHYV AFVKYGKDDSAWLFFDSMADRDGGQ NGFNIPQVTPCPEVGEYLKMSLEDL HSLDSRRIQGCARRLLCDAYMCMYQ SPTMSLYK
Stability : 12 months at -70 centigrade; aliquot as required
Storage : At -70 centigrade
Concentration : 0.5 mg/ml
Storage Buffer : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Gene Name : CYLD CYLD lysine 63 deubiquitinase [ Homo sapiens (human) ]
Official Symbol : CYLD
Synonyms : CYLD; CYLD lysine 63 deubiquitinase; EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2; ubiquitin carboxyl-terminal hydrolase CYLD; cylindromatosis (turban tumor syndrome); deubiquitinating enzyme CYLD; probable ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; ubiquitin-specific-processing protease CYLD; EC 3.4.19.12
Gene ID : 1540
mRNA Refseq : NM_015247
Protein Refseq : NP_056062
MIM : 605018
UniProt ID : Q9NQC7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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