Recombinant Human CYLD Protein, His-tagged CYLD-22H

Recombinant Human CYLD Protein, His-tagged

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Recombinant Human CYLD Protein, His-tagged

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Cat.No. : CYLD-22H
Product Overview : Recombinant Human CYLD Protein with His tag was expressed in sf21 insect cell-baculovirus.
Description : This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Source : Insect Cell
Species : Human
Tag : His
Bio-activity : Deubiquitylating Enzyme Assay: The activity of His-CYLD was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-CYLD was compared confirming the deubiquitylating activity of His-CYLD.
Molecular Mass : ~110 kDa
AA Sequence : MSYYHHHHHH DYDIPTTENL YFQGAMGSSS GLWSQEKVTS PYWEERIFYL LLQECSVTDK QTQKLLKVPK GSIGQYIQDR SVGHSRIPSA KGKKNQIGLK ILEQPHAVLF VDEKDVVEIN EKFTELLLAI TNCEERFSLF KNRNRLSKGL QIDVGCPVKV QLRSGEEKFP GVVRFRGPLL AERTVSGIFF GVELLEEGRG QGFTDGVYQG KQLFQCDEDC GVFVALDKLE LIEDDDTALE SDYAGPGDTM QVELPPLEIN SRVSLKVGET IESGTVIFCD VLPGKESLGY FVGVDMDNPI GNWDGRFDGV QLCSFACVES TILLHINDII PALSESVTQE RRPPKLAFMS RGVGDKGSSS HNKPKATGST SDPGNRNRSE LFYTLNGSSV DSQPQSKSKN TWYIDEVAED PAKSLTEIST DFDRSSPPLQ PPPVNSLTTE NRFHSLPFSL TKMPNTNGSI GHSPLSLSAQ SVMEELNTAP VQESPPLAMP PGNSHGLEVG SLAEVKENPP FYGVIRWIGQ PPGLNEVLAG LELEDECAGC TDGTFRGTRY FTCALKKALF VKLKSCRPDS RFASLQPVSN QIERCNSLAF GGYLSEVVEE NTPPKMEKEG LEIMIGKKKG IQGHYNSCYL DSTLFCLFAF SSVLDTVLLR PKEKNDVEYY SETQELLRTE IVNPLRIYGY VCATKIMKLR KILEKVEAAS GFTSEEKDPE EFLNILFHHI LRVEPLLKIR SAGQKVQDCY FYQIFMEKNE KVGVPTIQQL LEWSFINSNL KFAEAPSCLI IQMPRFGKDF KLFKKIFPSL ELNITDLLED TPRQCRICGG LAMYECRECY DDPDISAGKI KQFCKTCNTQ VHLHPKRLNH KYNPVSLPKD LPDWDWRHGC IPCQNMELFA VLCIETSHYV AFVKYGKDDS AWLFFDSMAD RDGGQNGFNI PQVTPCPEVG EYLKMSLEDL HSLDSRRIQG CARRLLCDAY MCMYQSPTMS LYK
Stability : 12 months at -70 centigrade; aliquot as required
Storage : At -70 centigrade
Concentration : 0.5 mg/ml
Storage Buffer : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Purity :

4-12% gradient SDS-PAGE
InstantBlue™ staining
Lane 1: MW markers
Lane 2: 1 µg His-CYLD

Gene Name : CYLD CYLD lysine 63 deubiquitinase [ Homo sapiens (human) ]
Official Symbol : CYLD
Synonyms : CYLD; CYLD lysine 63 deubiquitinase; EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2; ubiquitin carboxyl-terminal hydrolase CYLD; cylindromatosis (turban tumor syndrome); deubiquitinating enzyme CYLD; probable ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; ubiquitin-specific-processing protease CYLD; EC 3.4.19.12
Gene ID : 1540
mRNA Refseq : NM_015247
Protein Refseq : NP_056062
MIM : 605018
UniProt ID : Q9NQC7

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