Active Recombinant Human CYLD Protein, His-tagged
Cat.No. : | CYLD-22H |
Product Overview : | Recombinant Human CYLD Protein with His tag was expressed in sf21 insect cell-baculovirus. |
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- Gene Information
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Description : | This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Source : | Insect Cell |
Species : | Human |
Tag : | His |
Bio-activity : | Deubiquitylating Enzyme Assay: The activity of His-CYLD was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-CYLD was compared confirming the deubiquitylating activity of His-CYLD. |
Molecular Mass : | ~110 kDa |
AA Sequence : | MSYYHHHHHHDYDIPTTENLYFQGA MGSSSGLWSQEKVTSPYWEERIFYL LLQECSVTDKQTQKLLKVPKGSIGQ YIQDRSVGHSRIPSAKGKKNQIGLK ILEQPHAVLFVDEKDVVEINEKFTE LLLAITNCEERFSLFKNRNRLSKGL QIDVGCPVKVQLRSGEEKFPGVVRF RGPLLAERTVSGIFFGVELLEEGRG QGFTDGVYQGKQLFQCDEDCGVFVA LDKLELIEDDDTALESDYAGPGDTM QVELPPLEINSRVSLKVGETIESGT VIFCDVLPGKESLGYFVGVDMDNPI GNWDGRFDGVQLCSFACVESTILLH INDIIPALSESVTQERRPPKLAFMS RGVGDKGSSSHNKPKATGSTSDPGN RNRSELFYTLNGSSVDSQPQSKSKN TWYIDEVAEDPAKSLTEISTDFDRS SPPLQPPPVNSLTTENRFHSLPFSL TKMPNTNGSIGHSPLSLSAQSVMEE LNTAPVQESPPLAMPPGNSHGLEVG SLAEVKENPPFYGVIRWIGQPPGLN EVLAGLELEDECAGCTDGTFRGTRY FTCALKKALFVKLKSCRPDSRFASL QPVSNQIERCNSLAFGGYLSEVVEE NTPPKMEKEGLEIMIGKKKGIQGHY NSCYLDSTLFCLFAFSSVLDTVLLR PKEKNDVEYYSETQELLRTEIVNPL RIYGYVCATKIMKLRKILEKVEAAS GFTSEEKDPEEFLNILFHHILRVEP LLKIRSAGQKVQDCYFYQIFMEKNE KVGVPTIQQLLEWSFINSNLKFAEA PSCLIIQMPRFGKDFKLFKKIFPSL ELNITDLLEDTPRQCRICGGLAMYE CRECYDDPDISAGKIKQFCKTCNTQ VHLHPKRLNHKYNPVSLPKDLPDWD WRHGCIPCQNMELFAVLCIETSHYV AFVKYGKDDSAWLFFDSMADRDGGQ NGFNIPQVTPCPEVGEYLKMSLEDL HSLDSRRIQGCARRLLCDAYMCMYQ SPTMSLYK |
Stability : | 12 months at -70 centigrade; aliquot as required |
Storage : | At -70 centigrade |
Concentration : | 0.5 mg/ml |
Storage Buffer : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Gene Name : | CYLD CYLD lysine 63 deubiquitinase [ Homo sapiens (human) ] |
Official Symbol : | CYLD |
Synonyms : | CYLD; CYLD lysine 63 deubiquitinase; EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2; ubiquitin carboxyl-terminal hydrolase CYLD; cylindromatosis (turban tumor syndrome); deubiquitinating enzyme CYLD; probable ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; ubiquitin-specific-processing protease CYLD; EC 3.4.19.12 |
Gene ID : | 1540 |
mRNA Refseq : | NM_015247 |
Protein Refseq : | NP_056062 |
MIM : | 605018 |
UniProt ID : | Q9NQC7 |
Products Types
◆ Recombinant Protein | ||
CYLD-23H | Active Recombinant Human CYLD Protein, His-tagged | +Inquiry |
Cyld-2408M | Recombinant Mouse Cyld Protein, Myc/DDK-tagged | +Inquiry |
CYLD-3393H | Recombinant Human CYLD Protein, MYC/DDK-tagged | +Inquiry |
CYLD-696H | Recombinant Human CYLD Protein, His (Fc)-Avi-tagged | +Inquiry |
CYLD-2238H | Recombinant Human CYLD Protein, GST-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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