Active Recombinant Human CYP1B1 protein, His-tagged

• Cat.No.: CYP1B1-595H
• Product Overview: Active Recombinant Human CYP1B1 protein(Q16678)(374-516 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 374-516 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Cytochrome P450 family 1 subfamily B member 1 (CYP1B1) is an enzyme, Which belongs to the cytochrome P450 superfamily. CYP1B1 can catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. Besides, Heat Shock Protein family B (small) member 2 (HSPb2) has been identified as an interactor of CYP1B1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1B1 and recombinant human HSPb2, CYP1B1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPb2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1B1 and HSPb2 was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 17.6kDa
• Purity: > 97%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Gene Information

• Gene Name: CYP1B1 cytochrome P450, family 1, subfamily B, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP1B1
• Synonyms: CYP1B1; cytochrome P450, family 1, subfamily B, polypeptide 1; cytochrome P450, subfamily I (dioxin inducible), polypeptide 1 (glaucoma 3, primary infantile) , GLC3A; cytochrome P450 1B1; CP1B; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile); GLC3A; CYPIB1; P4501B1
• Gene ID: 1545
• mRNA Refseq: NM_000104
• Protein Refseq: NP_000095
• MIM: 601771
• UniProt ID: Q16678