Recombinant Human CYP27A1 protein, His-tagged

• Cat.No.: CYP27A1-3977H
• Product Overview: Recombinant Human CYP27A1 protein(183-531 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 183-531 aa
• AA Sequence: DDFMTRLDQLRAESASGNQVSDMAQ LFYYFALEAICYILFEKRIGCLQRS IPEDTVTFVRSIGLMFQNSLYATFL PKWTRPVLPFWKRYLDGWNAIFSFG KKLIDEKLEDMEAQLQAAGPDGIQV SGYLHFLLASGQLSPREAMGSLPEL LMAGVDTTSNTLTWALYHLSKDPEI QEALHEEVVGVVPAGQVPQHKDFAH MPLLKAVLKETLRLYPVVPTNSRII EKEIEVDGFLFPKNTQFVFCHYVVS RDPTAFSEPESFQPHRWLRNSQPAT PRIQHPFGSVPFGYGVRACLGRRIA ELEMQLLLARLIQKYKVVLAPETGE LKSVARIVLVPNKKVGLQFLQRQC
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: CYP27A1 cytochrome P450, family 27, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP27A1
• Synonyms: CYP27A1; cytochrome P450, family 27, subfamily A, polypeptide 1; CYP27, cytochrome P450, subfamily XXVIIA (steroid 27 hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1; sterol 26-hydroxylase, mitochondrial; cerebrotendinous xanthomatosis; CP27; CTX; cytochrome P450 27; sterol 27-hydroxylase; cytochrome P-450C27/25; vitamin D(3) 25-hydroxylase; cholestanetriol 26-monooxygenase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1; CYP27
• Gene ID: 1593
• mRNA Refseq: NM_000784
• Protein Refseq: NP_000775
• MIM: 606530
• UniProt ID: Q02318