| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Cytochrome P450 2E1 (abbreviated CYP2E1, EC 1.14.14.1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. |
| Specific Activity : |
Chlorzoxazone 6-hydroxylation:16.6 nmol/min/nmol of P450 at 37°C. Reconstitution conditions: 30 pmol CYP2E1, 60 pmol NADPH cytochrome P450 reductase, 30 pmol human cytochrome b5, 30 µg CHAPS, 6 µg liposomes {1:1:1 1,2-dilauroyl-sn-glycero-3-phosphocholine, 1,2-dileoyl-sn- glycero-3- phosphocholine, 1,2-dilauroyl-sn-glycero-3-phosphoserine}, 0.6 mM reduced glutathione, 10 mM HEPES/KOH (pH 7.4), and 1mM chlorzoxazone in a total reaction volume of 0.3 mL. Reactions started with the addition of 2 mM NADPH. N-nitrosodimethylamine demethylation: 17.2 nmol/min/nmol ofP450 at 37°C. Reconstitution conditions performed as abovewith 1.67X enzymes and lipids in a volume of 0.5 mL.N-nitrosodimethylamine concentration at 4 mM and reaction started with the addition of 2 mM NADPH. |
| Concentration : |
1.53 mg/mL total protein using the BCA protein assay with BSA as a standard. |
| Specific Content : |
16 nmol of spectral P450 per milligram of protein. |
| Storage Buffer : |
20 mM potassium phosphate (pH 7.4), 0.2 mM EDTA, 1 mM DTT, 20% (v/v) Glycerol. |
| Purity : |
Greater than 85% as assessed by visual inspection of a Coomassie® Blue-stained SDS-PAGE gel. |
| Storage : |
-80˚C long-term; -20˚C less than one month; avoid multiple freeze-thaw cycles. |
