Recombinant Human CYP2E1 protein, His-tagged

• Cat.No.: CYP2E1-32H
• Product Overview: Recombinant Human CYP2E1 fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: CYP2E1 also known as Cytochrome P450 2E1 (abbreviated CYP2E1, EC 1.14.13.n7), is member of the cytochrome P450 mixed-function oxidase system that involved in the metabolism of xenobiotics in the body. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid, In 20mM Tris-HCl (pH8.0) containing 10% glycerol
• Molecular Mass: 56.2kDa (488aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKDEFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILFRKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEKEKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVPSNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAILQHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS
• Purity: > 85% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP2E1
• Synonyms: CPE1; CYP2E; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase
• Gene ID: 1571
• mRNA Refseq: NM_000773
• Protein Refseq: NP_000764
• MIM: 124040
• UniProt ID: P05181
• Chromosome Location: 10q26.3
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Chemical carcinogenesis, conserved biosystem
• Function: arachidonic acid epoxygenase activity; enzyme binding; oxidoreductase activity