Recombinant Human CYP2E1 protein, MYC/DDK-tagged

• Cat.No.: CYP2E1-452H
• Product Overview: Recombinant Human CYP2E1 fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 56.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP2E1
• Synonyms: CPE1; CYP2E; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase
• Gene ID: 1571
• mRNA Refseq: NM_000773
• Protein Refseq: NP_000764
• MIM: 124040
• UniProt ID: P05181
• Chromosome Location: 10q26.3
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Chemical carcinogenesis, conserved biosystem
• Function: arachidonic acid epoxygenase activity; enzyme binding; oxidoreductase activity

Reviews

07/04/2022

Product is as described.

04/12/2022

Fast and efficient service.

12/19/2020

Great for biological experiments.

Q&As

Are there known genetic polymorphisms in CYP2E1 associated with altered drug metabolism or disease risk?

Genetic polymorphisms in CYP2E1 can result in altered enzyme activity, influencing an individual's response to drugs and susceptibility to liver damage.

What is the main function of CYP2E1 in drug metabolism?

CYP2E1 oxidizes and metabolizes various small molecules, including drugs and toxins.

How does CYP2E1 contribute to the metabolism of ethanol and other toxins?

It plays a significant role in ethanol metabolism and helps detoxify industrial solvents and other xenobiotics.

What factors can induce the expression of CYP2E1?

Ethanol, fasting, and certain drugs can induce the expression of CYP2E1.

How is CYP2E1 linked to the generation of reactive oxygen species (ROS)?

CYP2E1 activity generates ROS, contributing to oxidative stress and potential cellular damage.

In which tissues is CYP2E1 predominantly expressed?

CYP2E1 is highly expressed in the liver, which is the main site for drug metabolism.

What role does CYP2E1 play in drug-drug interactions?

CYP2E1 can contribute to drug-drug interactions, affecting the efficacy and toxicity of co-administered drugs.