| Cat.No. : |
CYP2J2-47H |
| Product Overview : |
Recombinant human CYP2J2 and human CYP-reductase without tag was coexpressed in Escherichia coli.
Amount: 0.5 nmol cytochrome P450 |
| Description : |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. |
| Source : |
E. coli |
| Species : |
Human |
| Bio-activity : |
Cytochrome c Reductase Activity: 353 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Terfenadine C-hydroxylase
Linear with time up to: 15 min(Determined at 1 µM substrate, using a P450 concentration of 0.125 pmol/ml)
Linear with P450 up to: 0.4 pmol/ml(Determined at 1 µM substrate, using an incubation time of 5 min)
Vmax: 33 pmol/min/pmol CYP
Km: 0.02 µM |
| Usage : |
For laboratory (research) purposes only. |
| Storage : |
Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
| Concentration : |
P450 Concentration: 1.0 nmol/ml
Protein Concentration: 10.0 mg/ml
Specific P450 Content: 100 pmol/mg protein |
| Storage Buffer : |
50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.