Recombinant Human CYP3A4 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CYP3A4-5369H
• Product Overview: CYP3A4 MS Standard C13 and N15-labeled recombinant protein (NP_059488) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 57.2 kDa
• AA Sequence: MALIPDLAMETWLLLAVSLVLLYLY GTHSHGLFKKLGIPGPTPLPFLGNI LSYHKGFCMFDMECHKKYGKVWGFY DGQQPVLAITDPDMIKTVLVKECYS VFTNRRPFGPVGFMKSAISIAEDEE WKRLRSLLSPTFTSGKLKEMVPIIA QYGDVLVRNLRREAETGKPVTLKDV FGAYSMDVITSTSFGVNIDSLNNPQ DPFVENTKKLLRFDFLDPFFLSITV FPFLIPILEVLNICVFPREVTNFLR KSVKRMKESRLEDTQKHRVDFLQLM IDSQNSKETESHKALSDLELVAQSI IFIFAGYETTSSVLSFIMYELATHP DVQQKLQEEIDAVLPNKAPPTYDTV LQMEYLDMVVNETLRLFPIAMRLER VCKKDVEINGMFIPKGVVVMIPSYA LHRDPKYWTEPEKFLPERFSKKNKD NIDPYIYTPFGSGPRNCIGMRFALM NMKLALIRVLQNFSFKPCKETQIPL KLSLGGLLQPEKPVVLKVESRDGTV SGATRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CYP3A4 cytochrome P450 family 3 subfamily A member 4 [ Homo sapiens (human) ]
• Official Symbol: CYP3A4
• Synonyms: CYP3A4; cytochrome P450, family 3, subfamily A, polypeptide 4; CYP3A3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450 3A4; nifedipine oxidase; cytochrome P450 3A3; cytochrome P450 HLp; cytochrome P450-PCN1; cytochrome P450 NF-25; albendazole sulfoxidase; quinine 3-monooxygenase; albendazole monooxygenase; P450-III, steroid inducible; glucocorticoid-inducible P450; taurochenodeoxycholate 6-alpha-hydroxylase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1; MGC126680
• Gene ID: 1576
• mRNA Refseq: NM_017460
• Protein Refseq: NP_059488
• MIM: 124010
• UniProt ID: P08684