Recombinant Human Cysteine Dioxygenase, Type I, His-tagged

• Cat.No.: CDO1-1250H
• Product Overview: CDO1 Human Recombinant fused with 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids (1-170 a.a.) and having a molecular mass of 23.9kDa. The CDO1 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Protein Length: 1-170 a.a.
• Description: CDO1 (Cysteine dioxygenase) is a mammalian non-heme iron enzyme that initiates a number of significant metabolic pathways associated with pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. CDO1 catalyzes the conversion of L-cysteine to cysteine sulfinic acid (cysteine sulfinate) by incorporation of dioxygen. CDO1 is a vital regulator of cellular cysteine concentrations and has an essential role in maintaining the hepatic concentration of intracellular free cysteine within a proper narrow range. CDO1 is able to alter intracellular cysteine levels and glutathione levels. CDO1 is highly expressed in the liver and placenta. On the other hand CDO1 has a low expression in heart, brain and pancreas. CDO1 can also be detected in hepatoblastoma HepG2 cells.
• Form: The CDO1 solution contains 20mM Tris buffer(pH 8.0), 10% glycerol and 1mM DTT.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile Filtered colorless solution.
• Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMEQ TEVLKPRTLA DLIRILHQLF AGDEVNVEEV QAIMEAYESD PTEWAMYAKF DQYRYTRNLV DQGNGKFNLM ILCWGEGHGS SIHDHTNSHC FLKMLQGNLK ETLFAWPDKK SNEMVKKSER VLRENQCAYI NDSVGLHRVE NISHTEPAVS LHLYSPPFDT CHAFDQR.
• Storage: CDO1 although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: CDO1 cysteine dioxygenase, type I [ Homo sapiens ]
• Official Symbol: CDO1
• Synonyms: CDO1; cysteine dioxygenase, type I; cysteine dioxygenase; EC 1.13.11.20; Cysteine dioxygenase type I; CDO-I; CDO
• Gene ID: 1036
• mRNA Refseq: NM_001801
• Protein Refseq: NP_001792
• MIM: 603943
• UniProt ID: Q16878.
• Chromosome Location: 5q23.2
• Pathway: Cysteine and methionine metabolism; Metabolic pathways; Taurine and hypotaurine metabolism
• Function: cysteine dioxygenase activity; ferrous iron binding; metal ion binding; oxidoreductase activity